93350
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93350
Sigma
Trizma® base
puriss. p.a., ≥99.7% (T)
| Synonym: | 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol |
| CAS Number: | 77-86-1 |
| Linear Formula: | NH2C(CH2OH)3 |
| Molecular Weight: | 121.14 |
| Beilstein Registry Number: | 741883 |
| EC Number: | 201-064-4 |
| MDL number: | MFCD00004679 |
| PubChem Substance ID: | 24889850 |
Description
| Other Notes | Easily compare specifications for Trizma products with the Trizma specification table. |
| The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. | |
| Legal Information | Trizma is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| grade | puriss. p.a. |
| assay | ≥99.7% (T) |
| ign. residue | ≤0.01% (as SO4) |
| pH | 10.5-11.5 (25 °C, 10% in H2O) |
| useful pH range | 7 - 9 |
| pKa (25 °C) | 8.1 |
| bp | 219-220 °C/10 mmHg(lit.) |
| mp | 167-172 °C(lit.) |
| 168-172 °C | |
| anion traces | chloride (Cl-): ≤5 mg/kg |
| sulfate (SO42-): ≤5 mg/kg | |
| cation traces | Ca: ≤10 mg/kg |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| λ | 10 % in H2O |
| UV absorption | λ: 230 nm Amax: 0.6 |
| λ: 260 nm Amax: 0.05 | |
| λ: 280 nm Amax: 0.04 | |
| λ: 430 nm Amax: 0.01 |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Gloves |
| Hazard Codes | Xi |
| Risk Statements | 36 |
| Safety Statements | 26 |
| WGK Germany | 2 |
| RTECS | TY2900000 |
References
| reference | Molloy, M.P., et al. Electrophoresis 19, 837, (1998) |
| Santoni, V., et al. Electrophoresis 21, 1054, (2000) | |
| Merck | Merck 13,9842 |
| Beilstein | Beil. 4,IV,1903 |
| Aldrich MSDS 1, 1815:C / Corp MSDS 1 (2), 3532:A / FT-IR 1 (1), 347:C / FT-IR 2 (1), 542:D / IR-Spectra (3), 203:G / IR-Spectra (2), 183:E / IR-Spectra (2), 183:D / IR-Spectra (3), 203:H / NMR-Reference 2 (1), 304:A / RegBook 1 (1), 371:C / Sax 6, 2558 / Sigma FT-IR 1 (2), 60:D / Sigma FT-IR 1 (2), 61:C / Structure Index 1, 52:C:5 / Vapor Phase 3, 437:C |
